The row started after the band refused permission for their song ‘Use Somebody’ to be included in the gay-friendly comedy.
Murphy called the band “self-centred assholes” and accused them of “missing the big picture”.
Kings Of Leon singer Caleb Followill said he was “shocked” by Murphy’s reaction and said it was normal practice to refuse licences when a song had been over-promoted.
But drummer Nathan Followill hit out on Twitter, writing “Dear Ryan Murphy, let it go. See a therapist, get a manicure, buy a new bra. Zip your lip and focus on educating 7yr olds how to say f*ck.”
He then added: “I’m sorry 4 anyone that misconstrued my comments as homophobic or misogynistic. I’m so not that kind of person. I really do apologize.”
Murphy fired back “Wow. That’s a homophobe badly in need of some education.”
He told gossip blog Perez Hilton: “I’m all for manicures, don’t wear a bra. Would guess most gay dudes don’t.
“But it’s telling that Nathan can reduce a group of people to a mean-spirited cliché, in a time where young gay men are killing themselves all over the country because of hatred like this.”